Interface or submerged slice pre-incubator with four rings
Description
The BSK5-4 Brain Slice Keeper has been designed to pre-incubate a large number of brain slices prior to transfer into recording chambers. Based on the BSK4, it consists of four closely fitting acrylic rings, installed into four holes on an upper manifold. The close fitting rings allow a removable sheet of nylon netting to be wedged and held in place. In this slice keeper, the slices can optionally be maintained either at an interface between the incubation medium and a humidified, high oxygen atmosphere within the holding vessel or fully submerged. Furthermore the slices can be maintained on a flat porous membrane (eg *Millicell-cm, 0.4um Cat.# PICMORG50 from Millipore) placed on the nylon netting in order to maintain the underside of slices perfectly flat for subsequent experiments utilizing microscope techniques.
Features
- Slices maintained for many hours either in interface or submerged mode with incubating media
- Four separate rings allow separation of different types of preparations
- Slices supported on a quick change nylon net with optional attached porous membrane
- Modular design simple to set up and maintain
Schematic
Schematic arrangement of BSK5-4 and location in purpose designed trough with conical profiled lid. Profile shows two of the four rings in cross section. Oxygen bubbles rise in the center carrying aCSF up and across beneath the surface of the slices, solution is re-directed towards the bubbler to create continuous circulation (green arrow). Approximate dimensions are in mm.
Downloads
BSk5-4 flyer – coming soon
References
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