Brain slice keeper with rings and net in place and with lid in
place (inset). A needle valve is used to control the flow of oxygen
mixture to the ceramic bubbler.
- Slices maintained for many
hours in incubating media
- Twelve separate rings allow
separation of different types of preparations
- Slices supported on a quick-change
- Modular design simple to
set up and maintain
The BSK12 Brain Slice Keeper has been designed to pre-incubate
a large number of brain slices prior to transfer into recording
chambers or for the incubation of preparations in experimental
test media. This keeper consists of twelve closely fitting acrylic
C rings that fit into their own through-hole, located
in a circular manifold. Removable netting is wedged between the
C ring and its hole. In operation, the twelve
rings located on a manifold with netting are totally immersed
in an ACSF-filled purpose designed holding vessel into which a
95% O2, 5% CO2 gas mixture is supplied through a ceramic bubbler.
The ceramic bubbler is located in a central tube chamber connected
to the upper manifold. The bubbles rising from the base saturate
the ACSF and provide constant circulation of medium to the slices
which rest on the nylon nets. Bubbles are restricted to rising
in the central chamber and are prevented from being trapped under
the slices, thereby ensuring a continuous circulation of medium.
Slices remain viable for many hours in these conditions. The BSK12
together with the holding vessel can be easily placed into a water
bath for regulating the incubating temperature as desired. A lid
with a conical profile ensures that condensation drops do not
fall directly above the slices. When in place, the fluid level
is maintained at least 3mm above the C rings. This
ensures circulation of fluid over the top and downwards towards
the slices. The typical fluid volume with BSK12 is 500ml but can
be reduced by adding large glass spheres into the base.
here for a flyer in PDF format.