- Slices maintained for many hours prior to transfer to recording
- Simple to set up and maintain
- Quick-change nylon net
The Brain Slice Keeper has been designed to pre-incubate brain slices
prior to transfer into recording chambers. It consists of a
pair of closely fitting acrylic rings, upper and lower, between
which is wedged a sheet of nylon netting ( see photo below).
In operation, the BSK1 is totally immersed in an ACSF-filled purpose designed holding vessel (BSKV) into which a 95% O2, 5% CO2 gas mixture is supplied with a ceramic
bubbler. The bubbles rising from the base of the BSKV saturate
the ACSF and provide constant circulation of medium to the slices
which rest on the nylon net. Since the lower ring of the BSK1
carries a deflector shield, bubbles are prevented from being
trapped under the slices and ensure continuous circulation of
medium. Slices remain viable for many hours in these conditions.
BSK1 together with the BSKV holding vessel can easily be placed
into a water bath for regulating the incubating temperature
as desired. The typical fluid volume with BSK1 plus BSKV is
here for a flyer in PDF format.
BSK1 slice keeper: carrier, netting and holding ring
BSK1 slice keeper and Holder BSKV: A
BSKV consists of a 200ml trough fitted with a ceramic air stone and needle
valve regulator for fine control of gas. B
View of BSKV from above. C BSKV with
BSK1 in place and D viewed from above.
of Brain Slice Keeper BSK1 and Holder BSKV.
Arrangement of BSK1 hooked into the holder BSKV.
Oxygen bubbles rising from the ceramic air stone A
induce circulation of aCSF to the top ring B
holding brain slices on the netting. Circulation continues down
on to the slices preventing movement and then out via open corners
of deflector at C and D.
The deflector has a curved half-cylinderical surface with closed
ends so that bubbles can not reach the underside of the net carrying
1999 Scientific Systems Design, Inc.