Home | Products | Prices | Contact | TCKG | TCKG Photo

Tissue Chamber - TCKG

      Click to view tckgphoto 
Applications
  • Isolated organ, skin, nerve and muscle preparations
  • Preparations maintained for many hours in flowing incubating media
  • Large diamond-shaped flow cell for maintaining preparations on a custom substrate
  • Grease-gap across a removable acrylic partition
  • Temperature controlled base heats incoming media flowing across preparation
  • Humidity and gas environment can be controlled within diamond-shaped flow cell
This chamber consists of two main cells, one is a large diamond-shape for containing the preparation and the other is a smaller oval-shaped cell where nerve ends are arranged for making electrical recordings. These two cells are separated by a removable acrylic partition. The bottom edge of this partition can be fashioned to leave a small arch through which nerve bundles can pass through for grease gap recording methods. The larger cell is normally pre-filled with an inert self-curing silicone rubber substrate to allow various types of preparations to be pinned in place. The smaller oval cell is supplied with a small pedestal on which a small pane of mirrored glass is used as a substrate to separate nerves from a nerve bundle with the aid of a dissecting microscope arranged over the cell. Hook-wire electrodes are arranged from nearby 1mm connectors for recording and stimulating functions as are ground electrodes for both nerve and preparation areas. Perfusion fluid flows into the chamber via a Teflon tube which first spirals in the base of the chamber. This base contains temperature control heating and sensor elements connected to a separately available proportional temperature controller PTC03. The warmed perfusion solution in the spiral then flows over the preparation in the diamond-shaped area and exits at the opposite end where it can be sucked off from a tube fitting. In addition there is provision for the diamond-shaped cell to maintain high humidity and control the gaseous environment for experimental anoxia or other gas combinations.

A flyer in PDF format can be viewed and printed.

LINE DRAWING TCKG

Feed solution enters through a plastic screw fitting A located on the top surface that connects to approx 30 inches of PTFE tubing to point B (connection not shown). The PTFE tubing spirals in the chamber base F which contains distilled water heated by a heater cartridge and connected to a temperature controller (PTC03). Warmed feed solution enters plastic fitting at C raised 5mm above main diamond shaped trough into which appropriate depth of Sylgard* or other silicone substrate will be set (by the end user). The Sylgard will not spill into the in / out ports as they are raised 5mm above the base. Solution is made to wick across the diamond Sylgard base with filter paper and reaches exit port D and then on to an exit plastic screw fitting E connected to a house waste vacuum line. Nerves are routed through a barrier located into grooves H-H (not shown) with a hole in it at the bottom. The nerve bundles are arranged on a raised mirror located in an oval area G and wire electrodes arranged from pin sockets on the surface.

*Sylgard is a registered trade name and trade mark of Dow Corning Corporation, USA

Home | Products | Prices | Contact | TCKG | TCKG Photo

Copyright© 1999 Scientific Systems Design, Inc.